Aubrey Moat V
Holmes
Summary
Neuroscience research technician with experience in neuroengineering, computational neuroscience, histology, and tissue engineering. Skilled in immunohistochemistry, cryosectioning, fluorescence microscopy, image analysis, and experimental support across collaborative academic research environments.
Overview
12
12
years of professional experience
Work History
Research Specialist A
University of Pennsylvania
Philadelphia, PA
01.2024 - Current
- Helped establish and operate histology and tissue-processing workflows supporting neuroengineering and translational neuroscience projects.
- Performed cryosectioning, immunohistochemistry, imaging, and tissue analysis of engineered neural tissue constructs.
- Processed FFPE porcine brain tissue using microtomy, H&E;, cresyl violet, and immunohistochemical staining workflows.
- Conducted vibratome and cryostat sectioning of rodent neural tissue for downstream imaging and analysis.
- Maintained histology instrumentation including cryostats and microtomes.
Research Specialist A
University of Pennsylvania
Philadelphia, PA
01.2021 - 01.2024
- Contributed to development of biohybrid neural interface technologies for auditory system restoration.
- Isolated and cultured primary rat cortical neurons for 2D and 3D engineered neural systems.
- Fabricated micro-TENN constructs and aggregate-based neuronal architectures.
- Performed neuronal culture maintenance, imaging, histological processing, and experimental support workflows.
- Designed and 3D printed custom laboratory equipment and fabrication tools.
Research Technician
University of Pennsylvania
Philadelphia, PA
01.2014 - 01.2016
- Participated in development of realistic compartmental models of retinal bipolar cells.
- Converted microscopy slides of retinal cells into 3D models and improved conversion workflows.
- Created equivalent circuit models and conducted simulated electrophysiology experiments.
- Tested models against published literature to ensure biological accuracy.
Education
Bachelor of Science - Neuroscience: Systems, Behavior & Plasticity / Psychology Minor
Temple University
Skills
- Tissue Engineering
- Cell Culture
- Microscopy
- Imaging
- Literature Search
- Histology
- Programming
- Digital Reconstruction
- Simulation
- CAD Design
Timeline
Research Specialist A
University of Pennsylvania
01.2024 - Current
Research Specialist A
University of Pennsylvania
01.2021 - 01.2024
Research Technician
University of Pennsylvania
01.2014 - 01.2016
Bachelor of Science - Neuroscience: Systems, Behavior & Plasticity / Psychology Minor
Temple University