Kathleen Miller
Lansdale,PA
Summary
Proactive and goal-oriented professional with excellent time management and problem-solving skills. Known for reliability and adaptability, with swift capacity to learn and apply new skills. Committed to leveraging these qualities to drive team success and contribute to organizational growth.
Overview
3
3
years of professional experience
Work History
Upstream Process Development Scientist
Astrix
04.2024 - Current
- Conducted fed-batch fermentation of E. coli in both 250 mL AMBR vessels and 10 L glass bioreactors (30 experiments)
- Conducted fed-batch fermentation of S. cerevisiae in stainless steel 15 L bioreactors (10 experiments)
- Supported development of a scalable plasmid DNA production platform
- Developed and implemented new strategies to improve efficiency of batching media in shake flasks
- Performed complex troubleshooting and assisted in leading initiatives to solve challenging scientific problems with instrumentation.
- Led media production and batching of shake flasks for all experiments
- Lab manager for 1 year
Research Technician I
Duke University Medical Center
07.2023 - 04.2024
- Conducted over 100 experiments related to molecular cloning, mouse behavior, protein purification, and proteomics
- Assisted in the design and implementation of new protocols and techniques
- Maintained accurate records of experiments, results, and observations
- Performed routine lab maintenance, including ordering supplies and ensuring a safe and clean work environment
Tutor
Widener University
08.2022 - 05.2023
- Helped over 25 students understand concepts in biology and psychology courses
- Answered questions and provided resources to students
Education
Bachelor of Science - Biology
Widener University
Chester, PA05-2023
Skills
- Laboratory techniques, safety processes and procedures, GMP, laboratory equipment functions, aseptic technique
- Ambr 250, 15 L stainless steel bioreactors, 10 L glass bioreactors
- Media production (reagents and solutions), formulation, batching
- Polymerase Chain Reactions (PCR), qPCR, DNA purification, genomic preps, pipetting, and serial dilutions
- Plasmid preparation (miniprep and maxiprep), QIAcube, cell culture, virus production, purification, and titer
- SDS-PAGE, western blotting, protein purification, GST pull downs, Bradford protein assays
- Software: Microsoft Office Suite, ELN, Signals, Spotfire, Power BI, DeltaV
- Experimental design, scientific writing, presentation, results analysis, data analysis
- Leadership, communication, critical thinking, problem solving
RESEARCH EXPERIENCE
- Undergraduate Research, Dept of Biochemistry Summer 2022-Spring 2023
- Genomic DNA isolation, PCR, gel extraction, sequence analysis on the UCSC genome browser
- Poster: Examination of CRISPR mutations in Zwischenferment gene in the model organism Drosophila melanogaster
- Presentations: SURCA Symposium, Widener University, 64th Annual Drosophila Research Conference, Chicago, IL
Timeline
Upstream Process Development Scientist
Astrix
04.2024 - Current
Research Technician I
Duke University Medical Center
07.2023 - 04.2024
Tutor
Widener University
08.2022 - 05.2023
Bachelor of Science - Biology
Widener University
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