Krysta Marie DiKun, Ph.D.
Bensalem
Overview
3
3
years of professional experience
Work History
Scientist I
Eurofins Lancaster Laboratories
06.2016 - 06.2017
- Plan logistics and carry out Validation study for the comparison between a compendial method of vaccine analysis and a method that utilizes DNA extraction and qPCR
- Perform Western Blot Assay for protein analysis of pre-sonicated vaccine samples
- Write and Second Scientist Review Technical Documents (Protocols, Reports, Communications)
- Uphold standards for performance and data recording in accordance with a GMP laboratory
Pharmacokinetic and Immune Response Research Assistant
Janssen Research
03.2015 - 09.2015
- Evaluated SPARCL platform for Pharmacokinetic application by comparison of assay with MSD and gyros through the optimization of capture/detect ratio, evaluation of matrix effects and assay stability, performance of a 40-plate batch size, automation of the assay on Hamilton Liquid Handler, and the evaluation of accuracy and precision of the newly automated assay
- Evaluated SPARCL platform for Immune Response application by comparison of assay with validated MSD assay through the optimization of capture/detect rations, the evaluation of dynamic range of ADA detectability/sensitivity/specificity, evaluation of drug tolerance, screening of high to low affinity ADAs, and evaluation of target sensitivity and tolerance
- Supported accurate record keeping and experimental data integrity in accordance with GMP/GLP laboratory practices
Molecular Pharmacology Research Assistant
Amicus Therapeutics
03.2014 - 09.2014
- Performed Single-Site PCR mutagenesis to create mutant forms of the IDUA gene to enhance enzyme replacement therapy for MPS I patients
- Transformed mutant forms into E.coli cells, cultured colonies, mini-prepped, and maxi- prepped mutations
- Transfected mutant forms into HEK-293 cells and conducted protein assays, enzyme activity assays, and Western Blots
- Performed Anti-GAA ELISA to evaluate antibody titer of Pompe patients
Education
Doctor of Philosophy (PhD) - Pharmacology And Genetics
Weill Cornell Graduate School of Medical Sciences
12.2023
B.S - Biological Sciences, Organismal Biology And Physiology
Drexel University
06.2016
Skills
- Assay Development
- Lab Experiments and Research
- Laboratory Techniques
- Data Analytics
- Critical Thinking
Publications
- (Accepted by PNAS – In Press) DiKun KM, Tang XH, Fu L, Choi ME, Lu C, Gudas LJ (2024). Retinoic Acid Receptor a Activity prevents kidney injury and fibrosis.
- DiKun KM, Gudas LJ. Vitamin A and retinoid signaling in the kidneys (2023). Pharmacology Therapeutics. PMID: 37331524.
- Gejman RS , Chang AY, Jones HF, DiKun K, Hakimi AA, Schietinger A, Scheinberg DA (2018). Rejection of immunogenic tumor clones is limited by clonal fraction. eLife 7: e141090.
Timeline
Scientist I
Eurofins Lancaster Laboratories
06.2016 - 06.2017
Pharmacokinetic and Immune Response Research Assistant
Janssen Research
03.2015 - 09.2015
Molecular Pharmacology Research Assistant
Amicus Therapeutics
03.2014 - 09.2014
Doctor of Philosophy (PhD) - Pharmacology And Genetics
Weill Cornell Graduate School of Medical Sciences
B.S - Biological Sciences, Organismal Biology And Physiology
Drexel University
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