Passionate and interest in childcare professional to contribute in early childhood development and education Committed to providing a safe, nurturing, and stimulating environment for children to grow and learn Seeking a position where I can utilize my skills and experience to make a positive impact on young lives
Premalatha Murugesan
Pittsburgh
Summary
Accomplished Biotechnologist with a proven track record at Shri Ramco Biotech, excelling in cell culture techniques and fostering collaborative teamwork. Demonstrated expertise in protein purification and quality control, significantly enhancing project outcomes. Skilled in leveraging time management capabilities to meet and exceed project goals efficiently.
Overview
1
1
year of professional experience
Work History
Biotechnologist
Shri Ramco Biotech
01.2008 - 06.2009
1. Preparation of Work Area
- Sterilize Work Area: Clean and sterilize the laminar flow hood and work surfaces using alcohol and other disinfectants.
- Prepare Personal Protective Equipment (PPE): Wear lab coat, gloves, and sometimes face masks to maintain a sterile environment.
- Weigh and Mix Ingredients: Accurately weigh and mix the components of the culture media (agar, nutrients, vitamins, etc.).
- Autoclave Media: Sterilize the prepared media using an autoclave to eliminate any contaminants.
- Pour Agar Plates: Pour the sterilized media into sterile containers or petri dishes.
- Select Plant Material: Choose healthy and disease-free plant parts (shoots, leaves, or buds) for culture.
- Surface Sterilize Plant Material: Treat plant material with disinfectants (like bleach or alcohol) to remove surface contaminants.
- Rinse and Dry: Rinse the plant material thoroughly with sterile distilled water and dry it using sterile filter paper.
- Transfer Plant Material: Under the laminar flow hood, transfer the sterilized plant material onto the prepared culture media.
- Label Containers: Properly label each container with relevant information (date, plant species, etc.).
- Place in Growth Chamber: Place the inoculated cultures in a growth chamber with controlled temperature, light, and humidity conditions.
- Monitor Growth: Regularly check the cultures for contamination and growth progress.
- Transfer to Fresh Media: When necessary, transfer growing cultures to fresh media to promote further growth.
- Maintain Records: Keep detailed records of each subculture, including dates and observations.
- Enter Data: Record data related to growth rates, contamination rates, and other observations in spreadsheets or lab notebooks.
- Analyze Data: Analyze the collected data to assess the success of the cultures and make necessary adjustments.
- Check Supplies: Regularly check and restock supplies like culture media, chemicals, and lab equipment.
- Order Reagents: Place orders for necessary reagents and supplies as needed.
- Follow SOPs: Adhere to standard operating procedures (SOPs) and safety guidelines to ensure compliance with regulations.
- Dispose Waste: Properly dispose of biological and chemical waste according to safety protocols.
- Team Meetings: Participate in team meetings to discuss progress, challenges, and new projects.
- Report Findings: Communicate findings and results to supervisors and colleagues.
Education
Master of Science - Biotechnology
Madurai Kamaraj University
Madurai05-2007
Bachelor of Science - Biotechnology
Bharathidasan University
Pudukkottai, Trichy05-2005
Skills
- Cell culture techniques
- Protein purification methods
- Time management capabilities
- Chromatography techniques
- Collaborative teamwork
- Quality control procedures
- Mentoring/Tutoring/Supervising
Interests
Timeline
Biotechnologist
Shri Ramco Biotech
01.2008 - 06.2009
Master of Science - Biotechnology
Madurai Kamaraj University
Bachelor of Science - Biotechnology
Bharathidasan University
Similar Profiles
Satya Sekhar GunnuSatya Sekhar Gunnu
Assistant Manager at Simran Shri Shri Pvt. Ltd.Assistant Manager at Simran Shri Shri Pvt. Ltd.
Bhavna SarkarBhavna Sarkar
Teacher at Shri Shri Ravishankar Vidya MandirTeacher at Shri Shri Ravishankar Vidya Mandir
Alok FalorAlok Falor
Hotel Manager at Shri Maheshwari Samaj Janupyogi Bhawan Abhinandan (unit Of Shri Maheshwari Samaj Jaipur)Hotel Manager at Shri Maheshwari Samaj Janupyogi Bhawan Abhinandan (unit Of Shri Maheshwari Samaj Jaipur)
Laura BednarLaura Bednar
Occupational Therapist at Advantage Care RehabilitationOccupational Therapist at Advantage Care Rehabilitation
Madeline KuzdzalMadeline Kuzdzal
Patient Care Technician at UPMC PresbyterianPatient Care Technician at UPMC Presbyterian