SUJATA PARAJULI
North Wales
Summary
Developed skills in collaborative research environment, excelling in data analysis and critical thinking. Seeking to transition into new field, leveraging strengths in research methodologies and scientific communication. Committed to applying detail-oriented approach to solve complex problems and drive innovation.
Overview
7
7
years of professional experience
Work History
Bioanalytical Scientist
Eurofins PSS Insourcing Solutions
02.2023 - Current
- Company Overview: Serving Merck & Co.
- Developed, optimized, and validated methods for assay development
- Conducted validation studies to ensure the accuracy, precision, and robustness of the developed methods
- Utilized automation platform to streamline assay development processes, resulting in improved efficiency and reproducibility
- Provided scientific laboratory support for the design and execution of customized immunoassays such as MSD assays, ELISpot, molecular and/or cell-based assays supporting licensed vaccine products
- Performed an electro chemiluminescent assay to measure the antibody concentrations from epidemiology studies
- Conducted neutralization assay development by using single cell imaging method such as Cytometer
- Supported the transfer of an assay in an automated platform
- Analyzed the data by using various statistical methods such as FORECAST, Interpolation, Interpolated Standard Curve, Graph Pad Prism, and Spotfire
- Maintained accurate records in electronic lab notebooks in adherence with documentation standards
- Trained new employees on areas of technical expertise and compliance issues relevant to lab setting.
Bench Scientist
Randstad Life Sciences
10.2020 - 12.2021
- Company Overview: Served Glaxo Smith Kline
- Maintained cell culture (cell lines / human primary cells) to support assay development
- Utilized various transfection/transduction methods to knockdown or overexpression target genes in cell lines primary cells
- Conducted experiments directed to investigate the biological effects of target genes
- Performed detections of biomarker changes using various assay methods, such as ELISA, Western, imaging, RT-PCR
- Analyzed and summarize experimental data using a variety of laboratory instrumentation and computer software
- Maintained accurate laboratory notebooks according to company guidelines
- Implemented quality control measures to ensure the accuracy and reliability of experimental results.
Graduate Research Assistant
Cabrini University
08.2019 - 05.2020
- Determined the cell surface phenotype of JMBD human lymphoid cell line using fluorescent antibody staining cytometry
- Isolated, Purified and Titration of monoclonal antibodies specific for human CD54
- Maintained, subculture and transfected COS7L cells (African Green Monkey kidney cell line) using electroporation and lipofection to introduce a reporter plasmid pEGFP-C
- Cultured a B cell hybridoma cell line, purified monoclonal antibodies produced by the hybridoma and used them to label a human erythroleukemia cell line(K562) with the antibodies
Associate Scientist
Fountain Group
11.2017 - 07.2018
- Company Overview: Served Merck & Co Inc
- Supported varicella bulk virus propagating using flow cytometry antigen expression assay
- Troubleshoot and worked independently to support the assay and conducted Co-Settling process
- Prepared laboratory reagents, solutions, and culture media
- Performed lab-scale upstream processing of vaccine products and process intermediates using aseptic techniques
- Analyzed, tracked, and maintained records tests results following good laboratory practices
- Performed routine calculations and maintained appropriate logbook and notebooks
- Used and maintained laboratory equipment's such as Vi-cell to perform the cell count
- Maintained a safe and organized laboratory environment by adhering to best practices for chemical handling and waste disposal.
Education
Master of Science - Biological Science
Cabrini University
Radnor, PA05.2020
Bachelor of Science - Biological Science
Rosemont College
Rosemont, PA05.2017
Skills
- Mammalian cell culture
- IPSC cell culture
- Isolation of genomic DNA
- Extraction of genomic RNA
- PCR
- Western blot
- Southern blot
- RNA isolation
- Neutralization Assay development
- MSD assay
- ECL assays
- Gel Electrophoresis
- SDS-PAGE
- ELISA
- Immunostaining
- Gene cloning
- Ribosomal DNA Transformation
- Aseptic technique
- GMPs training
- MPS/Sbc introduction
- VFAE Assay Data analysis
- Statistical data analysis
- SAP/PASX Interface
- Real time Gating on Flow Cytometry
- Immunopurification with MACS technology
- Keyence fluorescence microscope
- Traditional Flow Cytometry
- FACS Caliber flow cytometer
- Guava Machine
- Cytometer
- Blue Cat Plate washer
- Ensight Plate Reader
- MSD Sector S 600
- Integra Biosciences ASSIST PLUS base unit
- Vi-cell
- NC200 cell counter
- Biomek
- Telios Software
- Momentum software
- Cytomat
- Gviz
- Veggie Uber Table
- Shotgun genome sequencing
- Sanger sequencing
- BLAST
- Bootstrap test
- Glimmer
- Gene Mark
- Phamerator
- PECAAN
- Galaxy
- R
- R studio
- Statistical methods
- T-test
- ANOVA test
- Chi square test
- Win List
- Track wise
- Quality Data Interchange
- Graph Pad Prism
- Spotfire
- Excel
- PowerPoint
Timeline
Bioanalytical Scientist
Eurofins PSS Insourcing Solutions
02.2023 - Current
Bench Scientist
Randstad Life Sciences
10.2020 - 12.2021
Graduate Research Assistant
Cabrini University
08.2019 - 05.2020
Associate Scientist
Fountain Group
11.2017 - 07.2018
Bachelor of Science - Biological Science
Rosemont College
Master of Science - Biological Science
Cabrini University
Similar Profiles
Camelia EldawyCamelia Eldawy
Downstream Development Scientist at Eurofins PSS Insourcing SolutionsDownstream Development Scientist at Eurofins PSS Insourcing Solutions
Kelsey ArcherKelsey Archer
Analytical Chemist II at Eurofins PSS Insourcing SolutionsAnalytical Chemist II at Eurofins PSS Insourcing Solutions
Lisa GillettLisa Gillett
GMP Investigation Specialist (Consultant) at Eurofins PSS Insourcing Solutions, LLCGMP Investigation Specialist (Consultant) at Eurofins PSS Insourcing Solutions, LLC